Born in London 8 March 1961, and raised in Canterbury, Kent in England. Padmore studied clarinet and piano prior to his gaining a choral scholarship to King's College, Cambridge. He graduated in 1982 with an honours degree in music.

He was first recognised as a singer with potential by William Christie and Philippe Herreweghe, with whom he sang several pieces written by Bach. His operatic experience is well-rounded and extensive, performing songs from such operas as Don Giovanni, Jephtha, and Platée. He has performed at various festivals, including the Edinburgh Festival and the Festival d'Aix-en-Provence.